Replication-Dependent Loss of 5-Hydroxymethylcytosine in Mouse Preimplantation Embryos
Fig. 1. Replication-dependent loss of 5- hydroxymethylcytosine during mouse preimplantation development. Representative confocal microscopy images of mitotic chromosome spreads derived from IVF mouse embryos co-stained with 5hmC (red), 5mC (green) antibodies or DAPI (blue) at 1-cell (A), 2-cell (B), 4-cell (C), and 8-cell (D) stages. Shown are the images of chromosomes from one blastomere at each developmental stage. As a result of sister chromatid exchange, only part of the sister chromatids stained positively for 5hmC at the 4-cell and 8-cell stages (C, D). The dotted squares represent the enlarged paternal (Pat.) or maternal (Mat.) chromosomes, respectively.